核酸基礎(chǔ)數(shù)據(jù)
一個(gè)DNA堿基對(duì)(鈉鹽)的平均分子量 = 650 道爾頓
1.0 A260 unit ds DNA = 50 μg/ml = 0.15 mM (in nucleotides)
1.0 A260 unit ss DNA = 33 μg/ml = 0.10 mM (in nucleotides)
1.0 A260 unit ss RNA = 40 μg/ml = 0.11 mM (in nucleotides)
雙鏈DNA分子的分子量(道爾頓) = 堿基對(duì)數(shù)目×650
雙鏈DNA分子的末端摩爾數(shù) = 2 ×DNA質(zhì)量(克)/ DNA分子量(道爾頓)
限制性內(nèi)切酶酶切后的DNA末端摩爾數(shù):
a) 環(huán)狀DNA分子: 2 × DNA摩爾數(shù)× 位點(diǎn)數(shù)
b) 線性DNA分子: 2 × DNA摩爾數(shù)×位點(diǎn)數(shù) 2 × DNA摩爾數(shù)
1 μg 1000 bp DNA = 1.52 pmol = 9.1 × 1011 molecules
1 μg pUC18/19 DNA (2686 bp) = 0.57 pmol = 3.4 × 1011 molecules
1 μg pBR322 DNA (4361 bp) = 0.35 pmol = 2.1 × 1011 molecules
1 μg M13mp18/19 DNA (7249 bp) = 0.21 pmol = 1.3 × 1011 molecules
1 μg λDNA (48502 bp) = 0.03 pmol = 1.8 ×1010 molecules
1 pmol 1000 bp DNA = 0.66 μg
1 pmol pUC18/19 DNA (2686 bp) = 1.77 μg
1 pmol pBR322 DNA (4361 bp) = 2.88 μg
1 pmol M13mp18/19 DNA (7249 bp) = 4.78 μg
1 pmol λDNA (48502 bp) = 32.01 μg
1.0 kb DNA = 333個(gè)氨基酸的編碼量≈ 37,000 道爾頓蛋白質(zhì)
10,000道爾頓蛋白質(zhì)≈270 bp DNA
50,000道爾頓蛋白質(zhì)≈ 1.35 kb DNA
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